aidsВИЧ-инфекция и иммуносупрессииHIV Infection and Immunosuppressive Disorders2077-9828Baltic Medical Education Center10.22328/2077-9828-2021-13-3-52-60aids-659Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL STUDIESСостояние митохондрий CD4+ Т-лимфоцитов у ВИЧ/ВГС-коинфицированных пациентов с различной эффективностью восстановления иммунной системы при проведении антиретровирусной терапииState of CD4+ T-lymphocytes mitochondria in HIV/HCV coinfected patients with different efficiency of immune system restoration during antiretroviral therapyКоролевскаяЛ. Б.KorolevskayaL. B.

Королевская Лариса Борисовна — кандидат медицинских наук, научный сотрудник лаборатории экологической иммунологии

614081, г. Пермь, ул. Голева, 13

Korolevskaya Larisa Borisovna 

Perm

bioqueen@mail.ruСайдаковаЕ. В.SaidakovaE. V.

Сайдакова Евгения Владимировна — кандидат биологических наук, старший научный сотрудник лаборатории экологической иммунологии

614081, г. Пермь, ул. Голева, 13 

Perm

radimira@list.ruВласоваВ. В.VlasovaV. V.

Власова Виолетта Викторовна — лаборант лаборатории экологической иммунологии

614081, г. Пермь, ул. Голева, д. 13

Perm

violetbaudelaire73@gmail.comШмагельК. В.ShmagelC. V.

Шмагель Константин Владимирович — доктор медицинских наук, заведующий лабораторией экологической иммунологии

614081, г. Пермь, ул. Голева, д. 13 

Perm

shmagel@iegm.ru«Институт экологии и генетики микроорганизмов Уральского отделения Российской академии наук» — филиал Пермского федерального исследовательского центра Уральского отделения Российской академии наукРоссияInstitute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences — a branch of the Perm Federal Research Center of the Ural Branch of the Russian Academy of SciencesRussian Federation2021241020211335260Copyright © Королевская Л.Б., Сайдакова Е.В., Власова В.В., Шмагель К.В., 20212021Королевская Л.Б., Сайдакова Е.В., Власова В.В., Шмагель К.В.Korolevskaya L.B., Saidakova E.V., Vlasova V.V., Shmagel C.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://hiv.bmoc-spb.ru/jour/article/view/659

Цель работы: оценка параметров митохондрий CD4+ Т-лимфоцитов у ВИЧ/ВГС коинфицированных пациентов с дискордантным и стандартным ответом иммунной системы на антиретровирусную терапию.Материалы и методы. Обследованы ВИЧ/ВГС коинфицированные пациенты с дискордантным (n=21) и стандартным (n=20) ответом на лечение. Контрольную группу составили 23 неинфицированных добровольца. Методом проточной цито - флюорометрии в CD4+ Т-лимфоцитах было установлено содержание PGC-1a, определены масса и мембранный потенциал митохондрий.Результаты. Показано, что величина митохондриального мембранного потенциала CD4+ Т-лимфоцитов у ВИЧ/ВГС коинфицированных субъектов со стандартным и дискордантным ответом на лечение сопоставима с таковой у неинфицированных доноров. У ВИЧ/ВГС коинфицированных больных по сравнению со здоровыми донорами повышена экспрессия PGC-1a и увеличена масса органелл в CD4+ Т-клетках. Установлено, что в отличие от здоровых лиц у ВИЧ/ВГС коинфицированных субъектов отсутствует зависимость между массой митохондрий и содержанием PGC-1a в CD4+ Т-лимфоцитах.Заключение. В CD4+ Т-клетках ВИЧ/ВГС коинфицированных пациентов с дискордантным и стандартным ответом иммунной системы на антиретровирусную терапию рост массы органелл не связан с производством энергии. При ВИЧ/ВГС коинфекции отсутствует взаимосвязь между массой митохондрий и регулятором их биогенеза.

Objective: to assess mitochondrial parameters in CD4+ T-cells of HIV/HCV coinfected patients with a discordant and standard response of the immune system to antiretroviral therapy.Materials and methods. HIV/HCV coinfected patients with discordant (n=21) and standard (n=20) response to treatment were examined. The control group comprised of 23 uninfected volunteers. In CD4+ T-cells, PGC-1a content, mitochondrial mass, and mitochondrial membrane potential were determined with flow cytometry.Results. In CD4+ T-cells of HIV/HCV coinfected subjects with standard and discordant response to treatment, mitochondrial membrane potential was similar to that of uninfected donors. Compared with healthy controls, HIV/HCV coinfected patients had increased organelles’ mass and PGC-1a expression in CD4+ T-cells. In contrast to healthy individuals, HIV/HCV coinfected subjects had no correlation between mitochondrial mass and PGC-1a content in CD4+ T-lymphocytes.Conclusion. In CD4+ T-cells of HIV/HCV coinfected patients with discordant and standard response to antiretroviral therapy, up-regulation of mitochondrial mass is not associated with energy production. In HIV/HCV coinfection, there is no relationship between the mitochondrial mass and mitochondrial biogenesis regulator.

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The authors declare that there are no conflicts of interest present.