THE CIRCULATING RECOMBINANT FORM RF2k/1b OF HEPATITIS C VIRUS: PROBLEMS IN DIAGNOSTICS AND THERAPY
https://doi.org/10.22328/2077-9828-2016-8-1-42-52
Abstract
Study objective: To find out the causes of nonstandard results of hepatitis C virus genotyping using the test system Abbott RealTime HCV Genotype II. Materials and methods: 19 plasma samples from HCV/HIV patients showing nonstandard genotyping results were studied by sequencing the NS5B and 5’UTR/core regions of viral genomes. Genotyping kits manufactured by Vector-Best and Interlabservice were additionally used. Results: Patients were found to be infected with RF2k/1b HCV. RF2k/1b HCV was found in 6% of the co-infected HCV/HIV patients. Conclusion: The routinely used test systems targeted to only one fragment of HCV genome, i.e. to 5’UTR/core, erroneously identify the RF2k/1b recombinant as genotype 2. Data on antiviral therapy for RF2k/1b HCV suggest that therapeutic regimens recommended for genotype 2 HCV are inadequate for RF2k/1b cases.
About the Authors
N. Ye. Dementyeva
Saint-Petersburg Center for Control of AIDS and Infectious Diseases; Institute of Experimental Medicine
Russian Federation
Russian Federation
O. V. Kalinina
Pasteur Research Institute of Epidemiology, and Northwest Regional Center for Prevention and Control of AIDS and Infectious Diseases
Russian Federation
Russian Federation
O. O. Znoyko
AI.Yevdokimov Moscow State Medico-Stomatological University
Russian Federation
Russian Federation
N. A. Belyakov
First Pavlov State Medical University of Saint-Petersburg; Institute of Experimental Medicine; Pasteur Research Institute of Epidemiology, and Northwest Regional Center for Prevention and Control of AIDS and Infectious Diseases
Russian Federation
Russian Federation
A. B. Zhebrun
Pasteur Research Institute of Epidemiology, and Northwest Regional Center for Prevention and Control of AIDS and Infectious Diseases
Russian Federation
Russian Federation
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Review
For citations:
Dementyeva N.Ye., Kalinina O.V., Znoyko O.O., Belyakov N.A., Zhebrun A.B. THE CIRCULATING RECOMBINANT FORM RF2k/1b OF HEPATITIS C VIRUS: PROBLEMS IN DIAGNOSTICS AND THERAPY. HIV Infection and Immunosuppressive Disorders. 2016;8(1):42-52. (In Russ.) https://doi.org/10.22328/2077-9828-2016-8-1-42-52
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