ACCURACY OF HIV-1 SUBTYPE ASSIGNMENT OF V3 LOOP GP120 GENE
https://doi.org/10.22328/2077-9828-2015-7-1-40-44
Abstract
The use of HIV-1 genotyping for tropism prediction before Maraviroc (MVC, Pfizer) prescription in Russia will produce a large number of env gene sequences. These data may be useful for both therapy and epidemiological surveillance. But HIV-1 subtyping tools performance on Russian strains is still undetermined. In this work we have investigated the accuracy of genotypic subtype prediction by Geno2pheno algorithm on env gene region. Concordant results between pol and env regions were for 84,7% samples. Agreement between phylogenetic analysis and automatic prediction by Geno2Pheno of env gene region was found in 90,6% that means quite high accuracy of the algorithm. Discordant results were associated with low sensitivity for recombinants and inaccurate algorithm performance.
Keywords
ВИЧ-1,
V3 петля,
gp120,
регион env,
субтипирование,
geno2pheno,
REGA,
HIV-1,
env gene,
subtyping,
Geno2pheno,
REGA
About the Authors
M. Y. Dmitryukova
Central Research Institute for Epidemiology
Russian Federation
Russian Federation
D. E. Kireev
Central Research Institute for Epidemiology
Russian Federation
Russian Federation
A. E. Lopatukhin
Central Research Institute for Epidemiology
Russian Federation
Russian Federation
I. A. Lapovok
Central Research Institute for Epidemiology
Russian Federation
Russian Federation
G. A. Shipulin
Central Research Institute for Epidemiology
Russian Federation
Russian Federation
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Review
For citations:
Dmitryukova M.Y., Kireev D.E., Lopatukhin A.E., Lapovok I.A., Shipulin G.A. ACCURACY OF HIV-1 SUBTYPE ASSIGNMENT OF V3 LOOP GP120 GENE. HIV Infection and Immunosuppressive Disorders. 2015;7(1):40-44. (In Russ.) https://doi.org/10.22328/2077-9828-2015-7-1-40-44
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